Effectiveness of Papaya Leaf Squeeze (Carica Papaya L.) as a biolarvicide against the larval mortality of mosquito larvae of Tribus Anophelini (Anopheles sp) and Tribus culicini (Aedes sp)
Keywords:
Papaya Leaves, Biolarviccide, Mortality, Anopheles sp, Aedes sp
Abstract
Mosquito-borne diseases are still a public health problem. Mosquitoes of the Anophelini tribe (Anopheles sp) play a role in the transmission of malaria, while the Culicini tribe (Aedes sp) transmits dengue hemorrhagic fever (DHF). One of the safest larval control efforts is the use of biolarviccides, such as papaya plants (Carica papaya L.) which are more friendly to humans and the environment. The purpose of the study was to determine the effectiveness of papaya leaf juice as a biolarvicide against the mortality of Anopheles sp and Aedes sp mosquito larvae. Pseudo-experimental research type, Non-equivalent control group design. Samples of 400 larvae, consisting of 200 larvae of Anopheles sp and 200 larvae of Aedes sp instar III. The larvae were given a concentration of 10%, 20%, and 30% papaya leaves with 3 repetitions, observed for 24 hours. Data analysis was conducted using the One-Way ANOVA test and the Independent Sample t-test. The results of the study were the highest at a concentration of 30% of 90%, the lowest at a concentration of 10% of 40%. The mortality of Aedes sp larvae is highest at a concentration of 30% at 95%, lowest at a concentration of 10% at 50%.
In conclusion, there was no difference in the effectiveness of papaya leaf juice as a biolarvicide on the mortality of Anopheles sp and Aedes sp mosquito larvae (p-value = 0.930). The public is advised to use papaya leaf juice biolarvicide, as an alternative to mosquito larval control that is more environmentally friendly.
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Alayda, N., Aidha, Z., & History, A. (2025). Analysis of the Implementation of the Malaria Control Program at the Gambir Baru Health Center. Journal of Preventive Promotion, 8(3), 419–433. http://journal.unpacti.ac.id/index.php/JPP
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Anggreini, Y. S., Agustarika, B., & Fabanyo, R. A. (2025). Community Empowerment through Mosquito Vector Control Education as an Effort to Prevent Dengue Disease and Malaria in the Community in Klamana Village. Journal of Community Service Creativity (PKM), 8(10), 4806–4819. https://doi.org/10.33024/jkpm.v8i10.22157
Arfah, A. M., Indasah, I., & Nurwijayanti, N. (2024). Education on Mosquito Vector Control as Disease Carriers in Students of SMAN 26 Central Maluku. Journal of Indonesian Innovation Community Service, 2(1), 163–168. https://doi.org/10.54082/jpmii.342
Bestari, R., Priawan F, Rosyidah D, & Cahyo F. (2020). Test of the Effectiveness of Betel Leaf Extract (Piper Betle L.) against Mortality of Anopheles Aconitus Larvae. Medika Respati : Scientific Journal of Health, 15.
Bisyaroh, N. (2020). Toxicity Test of Moringa Seed Extract (Moringa Oleifera) against Aedes Aegypti Mosquito Larvae. Journal of Pharmaceutical Tinctures, 1(2), 34–44. https://doi.org/10.35316/tinctura.v1i2.987
Dhenge, N. F., Feed, P. D., & Lidia, K. (2021). Larviccide Effectiveness Test of Papaya Leaf Extract (Carica papaya) on Larval Mortality of Dengue Hemorrhagic Fever Mosquito Aedes aegypti. Cendana Medical Journal, 21(1), 156–162.
Fahtori, F., Solikah, M. P., Anggraeni3, R., Study, P., & Applied, S. (2025). Comparison of the Effectiveness of Papaya Leaf Infestation with Temephos 1% as a Larvicide of Aedes Aegypti Mosquitoes That Cause Dengue. Indonesian Journal of Health Research Innovation (Ijhri), Vol 02 No(03), 113–123. https://journal.ymci.my.id/index.php/ijhri/index
Fajriansyah, F., & Sartika, I. (2022). Effect of Papaya Leaf Extract (Carica papaya linn) on Aedes aegypti Larvasida. Sago Journal of Nutrition and Health, 3(2), 157. https://doi.org/10.30867/gikes.v3i2.926
Handiny, F., Rahma, G., & Rizyana, N. (2020). Vector Control (N. Pangesti (ed.)). Ahlimedia Press.
Ministry of Health of the Republic of Indonesia. (2025a). Dengue Cases Reach 131 Thousand, 544 People Died. CNN Indonesia.
Ministry of Health of the Republic of Indonesia. (2025b). Malaria Cases in Indonesia. Ministry of Health.
Khalish, V. Al, Harminarti, N., & Katar, Y. (2025). Test the Potential of Malu's Putri Leaf Extract (Mimosa pudica Linn) Growing in Padang as a Vegetable Larvicide against Aedes aegypti Mosquito Larval Mortality. Andalas Health Journal, 9(2), 195–202. https://doi.org/10.25077/jka.v9i2.1250
Maksum, T. S., & Irwan, I. (2024). The Efficacy of Cymbopogon nardus ( Citronella ) Leaf Extract Biolarvicide on the Mortality of Third Instar Larvae of Malaria Vector. 7, 1110–1117. https://doi.org/10.26655/JMCHEMSCI.2024.9.1
Maulana, M., Hidayah, N., Nugrah, D., & Kusuma, I. K. (2022). Test the Effectiveness of Ethanol Extract of Papaya Leaves (Carica papaya Linn) as a Biolarvicide of Ae.aegypti. An-Nadaa: Journal of Public Health, 9(1), 14–21. https://ojs.uniska-bjm.ac.id/index.php/ANN/article/view/6060
Nimah, L., & Hidayati, L. (2020). Strengthening PHBS Plus in an effort to prevent tropical diseases and Covid-19 in Tambakwedi, Surabaya, East Java. Journal of Community Service, 16(2), 145–154.
Ningsi, E., Yuniar, N., & Fachlevy, A. (2016). Effectiveness of Killing Power Test of Papaya Leaf Extract (Carica papaya L.) Against Anopheles aconitus donits Mosquito Larvae in Efforts to Prevent Malaria in the Rice Field Area of Lalonggombu Village, Andoolo District, South Konawe Regency. Scientific Journal of Public Health Students Unsyiah, 1(3), 1–10.
Noviani, Prambudi, D. A., & Mulyadi, F. (2020). The expert system for diagnosing diseases in papaya plants uses a web-based backward chaining method. Poltanesa Bulletin, 21(2), 50–51.
Nurhayani, N., Sari, D. N., Bestari, R. S., & Risanti, E. D. (2021). Test the Effectiveness of Tobacco Leaf Extract (Nicotiana tabacum L.) Against Mortality of Anopheles aconitus Larvae. Biomedicine, 13(1), 68–75. https://doi.org/10.23917/biomedika.v13i1.11272
Permatasari, D. (2024). Dengue Fever Risk Analysis on the Environment: Reviewing Impacts and Management Strategies. Al Ihsan Health Journal, 1(1), 1–6.
Ramayanti, I., & Febriani, R. (2016). Larvicide Effectiveness Test of Papaya Leaf Extract (Carica papaya Linn) against Aedes aegypti Larvae. Medicine, 6(2), 79–88. https://doi.org/10.32502/SM.V6I2.1383
Sampe, V., & Watuguly, T. (2016). Effectiveness of Pangi Seed Extract (Pangium edule Reinw) on Mortality of Anopheles Mosquito Larvae. 2(2), 152–159.
Sudarwati, T. P., & Fernanda, H. (2019). Application of the Utilization of Papaya Leaves (Carica papaya) as a Biolarvicide for Aedes aegypti Larvae (N. Hariyati (ed.)). Granite. www.penerbitgraniti.com
Tamba, I. G., Sudarmaja, I. M., Swastika, I. K., & Diarthini, N. L. (2023). Effectiveness of using papaya seed powder (Carica papaya L) as a larvicide of Aedes aegypti mosquito larvae. Digest of Medical Science, 14(1), 425–428. https://doi.org/10.15562/ism.v14i1.1703
Tunggadewi, A., Zulkarya, L., Nafi'ah, L., Handayani, Y., Wildayanti, Pratiwi, Y., Firmansyah, G., Pratitis, M., & Permatasari, N. I. (2025). Socialization on Dengue Prevention with the Utilization of Papaya Seeds as Larvicides to Overcome Mosquito Development at SMK DutaKarya. The Stage of Kindness: Journal of Social Service, 2(3), 33–37. https://doi.org/10.62951/panggungkebaikan.v2i3.1953
Utami, A. W., & Porusia, M. (2023). Literature Review of the Effect of Plant Insecticides and Synthetic Insecticides on the Larval Mortality of Aedes Aegypti Mosquito Larvae. Journal of Public Health, 11(2), 168–189. https://doi.org/10.14710/jkm.v11i2.37721
WHO. (2005). Guidelines For Laboratory and Field Testing of Mosquito Larvicides. In World Health Organization.
WHO. (2024). World Malaria Report 2024. World Health Organization.
WHO. (2025). Dengue. World Health Organization.
Widi, U., Mardawati, E., & Putri, S. H. (2020). Testing the Antioxidant Activity of Red Dragon Fruit Peel (Hylocereus polyrhizus) as a Peel Off Gel Mask. Journal of Agricultural Industry, 2(1), 95–102.
Published
2026-04-30
How to Cite
Putri Nurul Afni Kau, Herlina Jusuf, & Tri Septian Maksum. (2026). Effectiveness of Papaya Leaf Squeeze (Carica Papaya L.) as a biolarvicide against the larval mortality of mosquito larvae of Tribus Anophelini (Anopheles sp) and Tribus culicini (Aedes sp). International Journal of Health, Economics, and Social Sciences (IJHESS), 8(2), 1737~1743. https://doi.org/10.56338/ijhess.v8i2.10983
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