Environmental Sanitation and Household Rodent Presence Among Suspected Leptospirosis Cases: Findings from Makassar, Indonesia
Abstract
Introduction: Leptospirosis is a zoonotic disease caused by the bacterium Leptospira interrogans. Residential areas frequently experience poor environmental sanitation, including waste accumulation, stagnant water, and rodent activity around households. Such conditions compromise environmental hygiene and facilitate the survival of Leptospira bacteria. Poor sanitation increases community exposure to environmental sources of infection, thereby elevating the risk of leptospirosis. Exposure may occur through direct or indirect contact with contaminated water or soil, particularly when stagnant water is contaminated with rodent urine. This study aimed to assess household rodent presence among suspected leptospirosis cases and to examine its association with environmental sanitation conditions. Polymerase chain reaction (PCR) testing and GIS-based spatial mapping were incorporated to support diagnostic and spatial interpretation.
Methods: This study employed a quantitative, descriptive cross-sectional observational design. The sample comprised 20 households of suspected leptospirosis patients selected through purposive sampling. Data were collected through direct household observation using a structured checklist.
Results: Chi-square analysis indicated no statistically significant associations between sanitation indicators and signs of rodent presence, including stagnant water (p = 0.068), waste accumulation (p = 0.068), rodent entry pathways (p = 0.178), drainage condition (p = 0.305), and wastewater disposal systems (SPAL) (p = 0.136). All examined variables demonstrated p-values greater than 0.05, indicating no statistically significant associations with observable rodent indicators. Nevertheless, the SPAL variable showed a non-significant trend toward association (p = 0.136), although it did not reach the conventional 5% significance threshold.
Conclusion: No statistically significant relationships were identified between household sanitation conditions including stagnant water, waste accumulation, drainage condition, wastewater disposal systems (SPAL), and clean water availability and signs of rodent presence. To mitigate rodent-related environmental health risks, communities are encouraged to improve environmental hygiene through proper waste management, routine maintenance of drainage systems, and the implementation of household-level rodent control measures.
References
Ginting, Grace Karina Rim Br SI. Lingkungan, Perilaku Personal Hygiene, dan Pemakaian APD Terhadap Kejadian Leptospirosis. 2022;6(2):236-250. https://doi.org/10.15294/higeia.v6i2.53916
Inayah N, Dyah Y, Santik P. Kejadian Leptospirosis di Kabupaten Kebumen Tahun 2022. 2023;7(4):550-561. https://doi.org/10.15294/higeia.v7i4.70682
Cheng Q, Fattah RA, Susilo D, et al. Determinants of healthcare utilization under the Indonesian national health insurance system – a cross ? sectional study. BMC Health Serv Res. Published online 2025. https://doi.org/10.1186/s12913-024-11951-8
Turnier P Le, Oberlis M, Lavergne A, Epelboin L. Leptospirosis seroprevalence and exposure factors in three informal settlements of French Guiana : An opportunistic survey. Published online 2025:1-15. https://doi.org/10.1371/journal.pntd.0013764
Khaira Ilma, Mursid Raharjo MM. Department of Environmental Health, Master Program, Faculty of Public Health, Diponegoro University, Semarang, Indonesia. 2 Department of Epidemiology and Tropical Disease, Faculty of Public Health, Diponegoro University Semarang, Indonesia 3 Department o. 2022;03(02). https://doi.org/10.20473/jcmphr.v3i02.40660
Manyullei S, Amqam H, Rahmadanti SI. Identification of Leptospira Serovar in Leptospirosis Suspect Serum in Manggala Subdistrict , Makassar City. 2021;9:407-410. https://doi.org/10.3889/oamjms.2021.5316
Chery G, Francis L, Hunte S ann, Leon P. Epidemiology of human leptospirosis in Saint Lucia , 2010 – 2017. Published online 2020:2010-2017. https://doi.org/10.26633/RPSP.2020.160
Ningsih I, Wahid MH. Leptospirosis Ditinjau dari Aspek Mikrobiologi Leptospirosis in Microbiology Point of View. 2022;07(1):31-43. https://doi.org/10.33019/ekotonia.v7i1.3141
Subhaktiyasa PG. Determining population and sample: quantitative and qualitative research methodology approaches. 2024;9:2721-2731. https://doi.org/10.29303/jipp.v9i4.2657
Priambodo A, Nur AA, Sandri D, Ahmada NH, Septiandiani F. Training On The Use Of Software Arcgis And Avenza Maps In Spatial Data Management And Digital Map For Village Apparatus In Purbalingga District. 2023;5(2013):497-506. https://doi.org/10.25157/ag.v5i1.9824
Sayanthi Y, Susanna D. Pathogenic Leptospira contamination in the environment : a systematic review. Infect Ecol Epidemiol. 2024;14(1). https://doi.org/10.1080/20008686.2024.2324820
Sri Nuryati, Ratna Dian K. The Correlation between Physical Environmental Factors and the Occurrence of Leptospirosis. 2018;14(2):223-230. https://doi.org/10.15294/kemas.v14i2.13527
Nuha NF, Anisa N, Novitasari D. Literature Review: Relationship Of Environmental Risk Factors And The Incidence Of Leptospirosis In Settlements (2018–2023). 2023;15(3):235-246. https://doi.org/10.20473/jkl.v15i3.2023.235-246
Sayono S. Evaluation of Rat Density and the Associated Factors in Leptospirosis Endemic Areas: The First Report on the Use of BI-Index.; 2024. https://doi.org/10.20473/jkl.v16i3.2024.190-199
Arasy AA, Adi MS. Analysis of Environmental Factors that Influence the Existence of Rats Positive for Leptospira sp . in Kendari City. 2023;9:806-814. https://doi.org/10.29303/jppipa.v9iSpecialIssue.5441
Awoniyi AM, Barreto AM, Argibay HD, et al. Systematic surveillance tools to reduce rodent pests in disadvantaged urban areas can empower communities and improve public health. Sci Rep. Published online 2024:1-14. https://doi.org/10.1038/s41598-024-55203-5
Setiyani E, Martini M, Saraswati LD. The Presence Of Rat And House Sanitation Associated With Leptospira sp . Bacterial Infection In Rats ( A Cross Sectional Study In Semarang , Central Java Province , Indonesia ). 2018;06008:1-4. https://doi.org/10.1051/e3sconf/20183106008
Costa F, Ribeiro GS, Felzemburgh RDM, et al. Influence of Household Rat Infestation on Leptospira Transmission in the Urban Slum Environment. 2014;8(12). https://doi.org/10.1371/journal.pntd.0003338
Muñoz-zanzi C, Mason MR, Encina C, Astroza A. Leptospira Contamination in Household and Environmental Water in Rural Communities in Southern Chile. 2014;22:6666-6680. https://doi.org/10.3390/ijerph110706666
Feitosa I, Santos B. Pavement Inspection in Transport Infrastructures Using Unmanned Aerial Vehicles ( UAVs ). Published online 2024:1-25. https://doi.org/doi.org/10.3390/su16052207
Santos NDJ, Sousa E, Reis MG, Ko AI, Costa F. Rat infestation associated with environmental deficiencies in an urban slum community with high risk of leptospirosis transmission Infestação por roedores associada a fatores ambientais numa comunidade carente com alto risco de transmissão da leptospirose Infestación por roedores asociada a factores ambientales en una comunidad sin recursos con alto riesgo de transmisión de la leptospirosis. 2017;33(2):1-13. https://doi.org/10.1590/0102-311X00132115
Ercumen A, Pickering AJ, Kwong LH, et al. Do Sanitation Improvements Reduce Fecal Contamination of Water, Hands, Food, Soil, and Flies? Evidence from a Cluster-Randomized Controlled Trial in Rural Bangladesh. Published online 2018. https://doi.org/10.1021/acs.est.8b02988
Of E, Density RAT, Associated The, In F, Endemic L. Evaluation Of Rat Density And The Associated Factors In Leptospirosis Endemic. 2024;16(3):190-199. https://doi.org/10.20473/jkl.v16i3.2024.190-199
Manyullei S, Natsir MF, Batkunda A. Identification of Rat Density and Ectoparasites in Seaport Area of Manokwari , Papua Province. 2020;8:204-208.
Zulaeka P, Manyullei S, Ibrahim E, Mallongi A, Saleh LM. Effectiveness of Management Quality and Information on the Environmental Health Program Monitoring Application ( TEMU-KL ) at The Baebunta Health. 2024;12(3):533-542. https://doi.org/10.13189/ujph.2024.120311
Wikurendra EA, Rangga JU, Yekti A, Asih P, Sinulingga TS. Polymerase chain reaction-driven detection of Leptospira spp. in urban rat populations of Surabaya, Indonesia. IJID One Heal. 2025;8(March):100074. https://doi.org/10.1016/j.ijidoh.2025.100074
Girikallo GG, Manyullei S, Ishak H, et al. Risk Factors for Schistosomiasis in East Lore District Poso Regency Central of Sulawesi Province, Indonesia. Univers J Public Heal. 2024;12(6):1070-1083. https://doi.org/10.13189/ujph.2024.120605
Manyullei S, Musbir AL, Natsir MF. Spatial Analysis of the Presence of Rats in three Student Housing Areas of Makassar City State Universities. 2025;13(1):54-62. https://doi.org/10.13189/ujph.2025.130105
Rafika A, Amqam H, Ibrahim E. Harnessing Liquid Smoke : Quality Evaluation of Organic Waste Combustion in Smokeless Incinerators. 2025;13(2):504-510. https://doi.org/10.13189/ujph.2025.130222
Melania A, Manyullei S, Ishak H, Amqam H, Hidayanty H, Syamsiar S. Open Access Community Based Biofiltration for Hard Water Treatment in Rural Indonesia : A Public Health Oriented Approach. Published online 2025:831-841. https://doi.org/10.56338/mppki.v8i9.7896
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