Pengaruh Pemberian Diet Tinggi Lemak Tinggi Gula terhadap Gambaran Histologi Hati Tikus Wistart Usia Kanak-Kanak
Effect of High Fat High Sugar Diet on Liver Histology of Wistart Rats of Childhood Agethe
Abstract
Latar belakang: Non Alcoholic Fatty Liver Disease (NAFLD) atau perlemakan hati non alcohol adalah penyakit degeneratif yang berkaitan erat dengan obesitas.
Tujuan: untuk melihat pengaruh pemberian diet tinggi lemak, tinggi gula terhadap gambaran hati tikust wistar usia kanak-kanak dengan kelompok kontrol. Serta perbandingan berat hati kelompok perlakuan dan kelompok kontrol.
Metode: penelitian eksperimental laboratorium dengan sampel penelitian 8 ekor Tikus Wistar berumur 8 minggu dengan berat 180 gram serta dilakukan adaptasi 2 minggu dan dibagi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol dengan pemberian pakan dan minuman secara ad libitum selama 7 minggu . Analisis data menggunakan uji Independent T-test.
Hasil: Rerata perbandingan berat hati lebih besar pada kelompok perlakuan (A) sebesar 12,75 gram dibandingkan kelompok kontrol 9,25 gram. Persentase kerusakan sel hati pada kelompok perlakuan lebih besar didandingkan kelompok kontrol dengan nilai rata-rata kelompok perlakuan (A) sebesar 91,10% dan kelompok kontrol (B) 4,07% (p = 0,000).
Kesimpulan: Pemberian diet tinggi lemak tinggi gula dapat mempengaruhi perubahan gambaran hati tikust wistart usia kanak-kanak dengan perbandingan persentase kerusakan tertinggi pada kelompok perlakuan dibdanding kelompok kontrol. Serta pemberian diet tinggi lemak tinggi gula mempengaruhi penambahan berat hati tertinggi pada kelompok perlakuan dibandingkan kelompok kontrol.
References
Suharidewi IGAT, Pinatih GI. GAMBARAN STATUS GIZI PADA ANAK TK DI WILAYAH KERJA UPT KESMAS BLAHBATUH II KABUPATEN GIANYAR TAHUN 2015. E-Jurnal Med Udayana [Internet]. 2017 Jun 15 [cited 2023 Jul 8];6(6). Available from: https://ojs.unud.ac.id/index.php/eum/article/view/31484
Kemenkes RI. Profil Kesehatan Indonesia 2018 [Internet]. Health Statistics. 2019. 207 p. Available from: https://www.kemkes.go.id/downloads/resources/download/pusdatin/profil-kesehatan-indonesia/profil-kesehatan-indonesia-2018.pdf
Setiyo P, Fakultas N, Masyarakat K, Muhammadiyah U, Timur K, No J, et al. JENIS KELAMIN DAN UMUR BERISIKO TERHADAP OBESITAS PADA REMAJA DI INDONESIA. An-Nadaa J Kesehat Masy [Internet]. 2020 Dec 19 [cited 2023 Jul 8];7(2):110–4. Available from: https://ojs.uniska-bjm.ac.id/index.php/ANN/article/view/3581
Zamzani M, Hadi H, Astiti D. Aktivitas fisik berhubungan dengan kejadian obesitas pada anak Sekolah Dasar. J Gizi dan Diet Indones (Indonesian J Nutr Diet [Internet]. 2017 May 22 [cited 2023 Jul 8];4(3):123–8. Available from: https://ejournal.almaata.ac.id/index.php/IJND/article/view/339
Sharma V, Coleman S, Nixon J, Sharples L, Hamilton-Shield J, Rutter H, et al. A systematic review and meta-analysis estimating the population prevalence of comorbidities in children and adolescents aged 5 to 18 years. Obes Rev [Internet]. 2019 Oct 1 [cited 2023 Jul 8];20(10):1341–9. Available from: https://pubmed.ncbi.nlm.nih.gov/31342672/
Ravertio D, Subroto T, Ketut Mariadi I, Somayana G, Made I, Dwipayana P. GAMBARAN PERLEMAKAN HATI NON-ALKOHOLIK DI RSUP SANGLAH DENPASAR PERIODE TAHUN 2017-2018. E-Jurnal Med Udayana [Internet]. 2019 Feb 19 [cited 2023 Jul 8];8(2):2019. Available from: https://ojs.unud.ac.id/index.php/eum/article/view/48563
Temple JL, Cordero P, Li J, Nguyen V, Oben JA. A Guide to Non-Alcoholic Fatty Liver Disease in Childhood and Adolescence. Int J Mol Sci [Internet]. 2016 Jun 15 [cited 2023 Jul 8];17(6). Available from: /pmc/articles/PMC4926480/
Bedossa P. Histological Assessment of NAFLD. Dig Dis Sci [Internet]. 2016 May 1 [cited 2023 Jul 8];61(5):1348–55. Available from: https://pubmed.ncbi.nlm.nih.gov/26874689/
Fang YL, Chen H, Wang CL, Liang L. Pathogenesis of non-alcoholic fatty liver disease in children and adolescence: From “two hit theory” to “multiple hit model.” World J Gastroenterol [Internet]. 2018 Jul 21 [cited 2023 Jul 8];24(27):2974–83. Available from: https://pubmed.ncbi.nlm.nih.gov/30038464/
Fan JG, Kim SU, Wong VWS. New trends on obesity and NAFLD in Asia. J Hepatol [Internet]. 2017 Oct 1 [cited 2023 Jul 8];67(4):862–73. Available from: https://pubmed.ncbi.nlm.nih.gov/28642059/
Wegermann K, Suzuki A, Mavis AM, Abdelmalek MF, Diehl AM, Moylan CA. Tackling Nonalcoholic Fatty Liver Disease: Three Targeted Populations. Hepatology [Internet]. 2021 Mar 1 [cited 2023 Jul 8];73(3):1199–206. Available from: https://pubmed.ncbi.nlm.nih.gov/32865242/
Corte C Della, Carpino G, De Vito R, De Stefanis C, Alisi A, Cianfarani S, et al. Docosahexanoic Acid Plus Vitamin D Treatment Improves Features of NAFLD in Children with Serum Vitamin D Deficiency: Results from a Single Centre Trial. PLoS One [Internet]. 2016 Dec 1 [cited 2023 Jul 8];11(12). Available from: /pmc/articles/PMC5158039/
Sari JI, Jusuf AA, Krisnuhoni E. Perbandingan Berat Hati Tikus Spraque Dawley yang Diberikan Diet Kurang Kalori, Cukup Kalori, dan Tinggi Kalori pada Masa Pertumbuhan. Alami J (Alauddin Islam Medical) J. 2018;2(2):15.
Softic S, Cohen DE, Kahn CR. Role of Dietary Fructose and Hepatic De Novo Lipogenesis in Fatty Liver Disease. Dig Dis Sci [Internet]. 2016 May 1 [cited 2023 Jul 8];61(5):1282–93. Available from: https://pubmed.ncbi.nlm.nih.gov/26856717/
Hassan N, Soliman G, Okasha E, Shalaby A. Histological, Immunohistochemical, and Biochemical Study of Experimentally Induced Fatty Liver in Adult Male Albino Rat and the Possible Protective Role of Pomegranate. J Microsc Ultrastruct [Internet]. 2018 [cited 2023 Jul 8];6(1):44. Available from: https://pubmed.ncbi.nlm.nih.gov/30023266/
Zhang Y, Ge S, Yang Z, Li Z, Gong X, Zhang Q, et al. Disturbance of di-(2-ethylhexyl) phthalate in hepatic lipid metabolism in rats fed with high fat diet. Food Chem Toxicol [Internet]. 2020 Dec 1 [cited 2023 Jul 8];146. Available from: https://pubmed.ncbi.nlm.nih.gov/33166671/
Piña-Zentella RM, Rosado JL, Gallegos-Corona MA, Madrigal-Pérez LA, García OP, Ramos-Gomez M. Lycopene Improves Diet-Mediated Recuperation in Rat Model of Nonalcoholic Fatty Liver Disease. J Med Food [Internet]. 2016 Jun 1 [cited 2023 Jul 8];19(6):607–14. Available from: https://pubmed.ncbi.nlm.nih.gov/27214602/
Asgharpour A, Cazanave SC, Pacana T, Seneshaw M, Vincent R, Banini BA, et al. A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol [Internet]. 2016 Sep 1 [cited 2023 Jul 8];65(3):579–88. Available from: https://pubmed.ncbi.nlm.nih.gov/27261415/
Zaki SM, Fattah SA, Hassan DS. The differential effects of high-fat and high- -fructose diets on the liver of male albino rat and the proposed underlying mechanisms. Folia Morphol (Warsz) [Internet]. 2019 [cited 2023 Jul 8];78(1):124–36. Available from: https://pubmed.ncbi.nlm.nih.gov/30009361/
Authors who publish with Journal of Public Health and Pharmacy retain the copyright of their work. The journal applies a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0), which grants the following rights:
-
Copyright Retention: Authors retain the copyright of their work, maintaining full control over their intellectual property without restrictions.
-
Right of First Publication: Authors grant the journal the right of first publication of their work. This ensures that the work is initially published and credited in Journal of Public Health and Pharmacy.
-
License to Share and Reuse: The work is licensed under CC BY-SA 4.0, allowing others to copy, distribute, remix, and build upon the work for any purpose, even commercially, as long as proper credit is given to the authors, and any new creations are licensed under the same terms.
