Identifikasi Gen ICSA pada Feses Anak Penderita Diare dengan Metode Polymerase Chain Reaction

  • Rusdianto Rusdianto Program Studi Pendidikan IPA Universitas Jember
  • Akbar Asfar Program Studi Ilmu Keperawatan Universitas Muslim Indonesia
  • Hairil Akbar Program Studi Kesehatan Masyarakat STIKES Graha Medika
DOI: https://doi.org/10.56338/mppki.v3i3.1135
Keywords: Diare, IcsA, PCR

Abstract

Gen IcsA merupakan salah satu gen yang berperan dalam virulensi pada bakteri Shigella sp yang dapat menyebabkan diare pada manusia. Penelitian ini bertujuan untuk mendeteksi gen IcsA  pada feses anak penderita diare dengan metode PCR.  Sebanyak 50 sampel klinis yang   diperoleh dari 4 puskesmas di Kota Makassar, yaitu Barabaraya, Pampang, Antang Perumnas dan Tamangapa dari bulan Mei 2016 - Juli  2016. Pengambilan sampel dilakukan dengan teknik rektal swab dan dilanjutkan pemeriksaan dengan  PCR menggunakan primer icsA. Hasil penelitian menunjukkan dari 50 sampel yang diuji diperoleh 6 sampel (24%) positif mengandung gen IcsA. Kesimpulan penelitian ini yaitu gen icsA efektif digunakan sebagai target gen (primer) untuk mendeteksi bakteri Shigella sp dengan metode PCR.

References

Walker, C.L.F., et al., Global burden of childhood pneumonia and diarrhoea. The Lancet, 2013. 381(9875): p. 1405-1416.

Organisation, W.G., World Gastroenterology Organisation global guidelines: acute diarrhea in adults and children: a global perspective. 2012, World Gastroenterology Organisation (WGO) Milwaukee, WI.

Farthing, M., et al., Acute diarrhea in adults and children: a global perspective. Journal of clinical gastroenterology, 2013. 47(1): p. 12-20.

Kemenkes RI., Data dan informasi tahun 2014 (profil kesehatan Indonesia). Kemenkes RI, Jakarta, 2015.

Kemenkes RI., Situasi diare di Indonesia. Buletin Jendela Data dan Informasi Kesehatan, 2011. 2(2): p. 1-6.

Sukut, S.S., Y. Arif, and N. Qur’aniati, Faktor kejadian diare pada balita dengan pendekatan teori Nola J. Pender di IGD RSUD Ruteng. Jurnal Pediomaternal, 2015. 3(2): p. 230-249.

Tjaniadi, P., et al., Antimicrobial resistance of bacterial pathogens associated with diarrheal patients in Indonesia. The American journal of tropical medicine and hygiene, 2003. 68(6): p. 666-670.

Nascimento, A.M. and S.T. Van Der Sand, Detection of the virulence plasmid pINV, using inv E gene in shigella sp. in sewage samples by PCR assay. Biociências (On-line), 2008. 16(1).

van der Ploeg, C.A., et al., Laboratory Protocol: Serotyping of Shigella spp. Geneva: WHO Global Foodborne Infections Network, 2010.

Binet, R., D.M. Deer, and S.J. Uhlfelder, Rapid detection of Shigella and enteroinvasive Escherichia coli in produce enrichments by a conventional multiplex PCR assay. Food microbiology, 2014. 40: p. 48-54.

Bryce, J., et al., WHO estimates of the causes of death in children. The Lancet, 2005. 365(9465): p. 1147-1152.

Sudaryat, S., Gastroenterologi Anak. Lab/SMF Ilmu Kesehatan Anak Fakultas Kedokteran. Universitas Udayana, 2010.

Brooks, G.F., J.S. Butel, and S.A. Morse, Jawetz, Melnick, & Adelberg Mikrobiologi Kedokteran, Edisi 23. Alih bahasa: Huriawati Hartanto et al. Editor edisi bahasa Indonesia: Retna Neary Elferia et al. Jakarta: Penerbit Buku Kedokteran EGC, 2008.

Levine, M.M., et al., Clinical trials of Shigella vaccines: two steps forward and one step back on a long, hard road. Nature Reviews Microbiology, 2007. 5(7): p. 540-553.

Wyllie, R., J.S. Hyams, and M. Kay, Pediatric gastrointestinal and liver disease. 2015: Elsevier Health Sciences.

Bhutta, Z.A., Acute gastroenteritis in children. Nelson textbook of pediatrics, 2007. 19: p. 1323-39.

Marcdante, K.J., et al., Nelson ilmu kesehatan anak esensial. 2014: Elsevier.

Nafianti, S. and A.B. Sinuhaji, Resisten Trimetoprim–Sulfametoksazol Terhadap Shigellosis. Sari Pediatri, 2005. 1(1): p. 39-44.

May, K.L. and R. Morona, Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. Journal of bacteriology, 2008. 190(13): p. 4666-4676.

Published
2020-09-05
How to Cite
Rusdianto, R., Asfar, A., & Akbar, H. (2020). Identifikasi Gen ICSA pada Feses Anak Penderita Diare dengan Metode Polymerase Chain Reaction. Media Publikasi Promosi Kesehatan Indonesia (MPPKI), 3(3), 178-182. https://doi.org/10.56338/mppki.v3i3.1135
Issue
Vol. 3 No. 3: SEPTEMBER 2020 - Media Publikasi Promosi Kesehatan Indonesia (MPPKI)
Section
Research Article

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