Identifikasi Gen ICSA pada Feses Anak Penderita Diare dengan Metode Polymerase Chain Reaction

  • Rusdianto Rusdianto Program Studi Pendidikan IPA Universitas Jember
  • Akbar Asfar Program Studi Ilmu Keperawatan Universitas Muslim Indonesia
  • Hairil Akbar Program Studi Kesehatan Masyarakat STIKES Graha Medika
DOI: https://doi.org/10.56338/mppki.v3i3.1135
Keywords: Diare, IcsA, PCR

Abstract

Gen IcsA merupakan salah satu gen yang berperan dalam virulensi pada bakteri Shigella sp yang dapat menyebabkan diare pada manusia. Penelitian ini bertujuan untuk mendeteksi gen IcsA  pada feses anak penderita diare dengan metode PCR.  Sebanyak 50 sampel klinis yang   diperoleh dari 4 puskesmas di Kota Makassar, yaitu Barabaraya, Pampang, Antang Perumnas dan Tamangapa dari bulan Mei 2016 - Juli  2016. Pengambilan sampel dilakukan dengan teknik rektal swab dan dilanjutkan pemeriksaan dengan  PCR menggunakan primer icsA. Hasil penelitian menunjukkan dari 50 sampel yang diuji diperoleh 6 sampel (24%) positif mengandung gen IcsA. Kesimpulan penelitian ini yaitu gen icsA efektif digunakan sebagai target gen (primer) untuk mendeteksi bakteri Shigella sp dengan metode PCR.

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Published
2020-09-05
How to Cite
Rusdianto, R., Asfar, A., & Akbar, H. (2020). Identifikasi Gen ICSA pada Feses Anak Penderita Diare dengan Metode Polymerase Chain Reaction. Media Publikasi Promosi Kesehatan Indonesia (MPPKI), 3(3), 178-182. https://doi.org/10.56338/mppki.v3i3.1135
Issue
Vol. 3 No. 3: SEPTEMBER 2020 - Media Publikasi Promosi Kesehatan Indonesia (MPPKI)
Section
Research Article

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