Acute and Lethal Dose Toxicity Test of Pure Eel Fish (Anguilla marmorata [Q.] Gaimard) Oil in Mice (Mus musculus)

  • Jamaluddin Jamaluddin Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
  • Ihwan Ihwan Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
  • Khildah Khaerati Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
  • Asriani Hasanuddin Department of Animal Husbandry, Faculty of Animal Husbandry and Fishery, Tadulako University, Palu, Central Sulawesi, 94148, Indonesia
  • Novalina Serdiati Department of Aquaculture, Faculty of Animal Husbandry and Fishery, Tadulako University, Palu, Central Sulawesi, 94148, Indonesia
  • Jusri Nilawati Department of Aquaculture, Faculty of Animal Husbandry and Fishery, Tadulako University, Palu, Central Sulawesi, 94148, Indonesia
  • Berniawan Wijaya Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
  • Yuliet Yuliet Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
  • Iftitah Ahmad Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jalan Soekarno-Hatta, Tondo, Palu City, Central Sulawesi, 94148, Indonesia
DOI: https://doi.org/10.56338/jphp.v5i3.5987
Keywords: Anguilla marmorata, Mus musculus, Acute Toxicity, Lethal Dose 50

Abstract

Introduction: Eel fish oil (Anguilla marmorata (Q.) Gaimard) highlighted its nutritional advantages, especially vitamin A, EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), and omega-3. Unsaturated fats in fish oil are known to have positive effects in the treatment of various diseases, although the potential negative impacts also need to be considered.

Methods: This research aims to focus on the cytotoxic activity of eel fish oil and carry out extraction using the soxhlation method, Thomson and Weil, and Metode fixed dose. To evaluate the toxicity of the extract, the acute toxicity test was carried out by measuring the middle lethal dose (LD50), which is often used as a benchmark. Apart from that, the research also aims to determine the lethal dose 50 (LD??) value of eel fish oil, namely the dose that can cause the death of 50% of the test animal population. The extraction process uses diethyl ether solvent at 60°C, followed by purification using a 3% bentonite adsorbent. The test was carried out twice with female mice and rats as test animals, divided into groups with oral doses of 5, 50, 300, 2000, and 5000 mg/kg.

Result: The results showed no deaths in the test animals, and the LD50 of eel fish oil extract was greater than the dose used. Clinical symptoms in mice include decreased motor activity and increased grooming frequency as the dose increases, as well as increased muscle tone and tension responses.

Conclusion: Based on the research results, it can be concluded that eel fish oil extracts up to a dose of 5000 mg/kg are still classified as "safe" for use because they do not cause death in test animals.

References

Sudaryono Agung. Sapto P. Putro S. Tinjauan Potensi Pengembangan dan Aplikasi Teknologi Budidaya Sidat. Semarang: Konferensi Akuakultur Indonesia. 2013.

Jamaluddin. Abu Bakar Putri A. Analisis Kadar Albumin Ikan Sidat (Anguilla marmorata Dan Anguilla bicolor) Dan Uji Aktivitas Penyembuhan Luka Terbuka Pada Kelinci (Oryctolagus cuniculus) Analysis of Albumin Levels of The Eels (Anguilla marmorata and Anguilla bicolor) And Examination Of. Galenika Journal of Pharmacy Journal of Pharmacy, 2016; 2(2), 90–95.

Somawathi K M. Rizliya V. Wijesinghe D G N G. & Madhujith W M T. Antioxidant activity and total phenolic content of different skin coloured brinjal (Solanum melongena). Tropical Agricultural Research, 2015; 26(1), 152. https://doi.org/10.4038/tar.v26i1.8080

Regulasi BPOM RI. Laporan Tahunan 2011 Badan Pengawas Obat dan Makanan. 2011.

Harmita. Mak R. Buku Ajar Analisis Hayati.Edisi 3.Penerbit Buku Kedokteran EGC: Jakarta. 2008; ISBN :978-979-448-875-1

BPOM regulations. Regulation of the Head of the Food and Drug Supervisory Agency of the Republic of Indonesia Number 17 of 2014 concerning Amendments to the Regulation of the Head of the Food and Drug Supervisory Agency Number HK.03.1.23.07.11.6662 of 2011 concerning Requirements for Microbial and Heavy Metal Contamination in Cosmetics. Jakarta: Republic of Indonesia Food and Drug Supervisory Agency. 2014.

Jamal Jamaluddin. Kadek N G A. Wulandari A S. Musnina W O S. & Widodo A. Effect of Solvent Type and Temperature Variation on Yield and Quality Parameters of Oil Extracted from Eel Fish (Anguilla marmorata [Q.] Gaimard) Using Soxhletation Method. Tropical Journal of Natural Product Research Available, 2021; 5(9), 1537–1541. https://doi.org/http://www.doi.org/10.26538/tjnpr/v5i9.3 (4)

Andhiarto Yanu. and Santi Wijaya. "Perbandingan Kualitas Suplemen Minyak Ikan Layang (Decapterus ruselli) Menggunakan Bentonit dengan Berbagai Konsentrasi pada Tahap Bleaching." Pharmacy: Jurnal Farmasi Indonesia, vol. 15, no. 02, 1 Dec. 2018, pp. 209-217,doi:10.30595/pharmacy.v15i2.3570. https://jurnal.unpad.ac.id/ijpst/article/view/7941/0 RI. Jakarta: Badan POM RI.

Alfiana Desinta, “Penganruh pengadukan terhadap ukuran partikel emulsi minyak ikan (Oleum iecoris aselli*).” p. skripsi, 2020.

Manggung R E R. Pengujian Toksisitas Akut Lethal Dose 50 (LD50) Ekstrak Etanol Buah Belimbing Wuluh (Avverrhoa billimbi) pada Mencit. Skripsi. Fakultas Kedokteran HewanInstitut Pertanian Bogor: Bogor. 2008; https:// repository. ipb.ac. id/handle / 123456789 /3430?show=full. Diakses pada tanggal 21 Mei 2021.

Purbonegoro T. Faktor-Faktor yang Mempengaruhi Toksisitas Bahan Pencemar terhadap organisme Perairan. Oseana. 2017; 42(2), pp.12-22.

Titiek B. Biomaker Toksisitas. In Airlangga University. 2017.

Nugroho RA. Aryani R. Manurung H. Rudianto R. Prahastika, W. Juwita A. Alfarisi A K. Pusparini N A O. and Lalong A. Acute and subchronic toxicity study of the ethanol extracts from Ficus deltoidea leaves in male mice. Open Access Macedonian Journal of Medical Sciences. 2020; 8(A), pp.76-83.

Mustapa M A. Tuloli T S. and Mooduto A M. Uji toksisitas akut yang diukur dengan penentuan LD50 ekstrak etanol bunga cengkeh (Syzygium aromaticum L.) terhadap mencit (Mus musculus) menggunakan metode thompson-weil. Frontiers: Jurnal Sains dan Teknologi. 2018; 1(1).

Alifa G.R. Uji Aktivitas Antiinflamasi Minyak Ikan Sidat (Anguilla bicolor) Pada Tikus Jantan Galur Wistar. Skripsi, 2017.

Kusumastuti N I. Uji Aktivitas Analgetik Minyak Ikan Sidat (Anguilla bicolor) Pada Mencit Putih (Mus musculus L.) Jantan Dengan Induksi Asam Asetat.Skripsi 2017.

Novianti. Aktivitas Antiinflamasi Ekstrak Minyak Ikan Sidat (Anguilla Marmorata) Fase Yellow Eel Asal Sungai Palu Pada Tikus Putih. Skripsi 2020.

Herza S. Inarah F. Ressi S. Test of the acute phase toxicity of toman fish (channa micropellets) fish extract oil on Wistar strain female rats using the OECD 425 method. (2022).

BPOM regulations. Food and Drug Supervisory Agency Regulation Number 10 of 2022 concerning Guidelines for In Vivo Preclinical Toxicity Testing.Republic of Indonesia Food and Drug Supervisory Agency, 2022; 1–220.

Lukiati B. Maslikhah S I. Potensi Ekstrak Etanol Labu Siam (Sechiumvedule) Untuk Terapi Tikus Wistar DM Hasil Induksi Streptozotocin [The potential of Ethanol Extract of chayote (Sechium edule) for the treatment of DM Wistar rats streptozotocin Induced]. Proceeding 1st National Research Symposium. Universitas Negeri Malang, 2014; 35-40.

Nurhayati E. Permatawati I. Kajian Awal Sintesis Biodiesel dari Minyak Dedak dan Metanol Melalui Ekstraksi dan Proses Esterifikasi. UNDIP, Semarang. 2009.

Tirpitz J L. Frieß U. Hendrick F. Alberti C. Allaart M. Apituley A. Bais A. Beirle, S. Berkhout S. Bognar K. and Bösch T. Intercomparison of MAX-DOAS vertical profile retrieval algorithms: studies on field data from the CINDI-2 campaign. Atmospheric Measurement Techniques Discussions, 2020; pp.1-49.

Alristina A D KM S. Ethasari R K. Gz S. Gz M. Laili R D. Hayudanti D. and Gz S. Ilmu gizi dasar buku pembelajaran. Penerbit CV. Sarnu Untung.2021.

Ayu. Characteristics of Fish Oil from Abdominal Fat by-product of Smoking Patin Fish (Pangasius hypophthalmus). Riau Province: Department of Fishery Products Technology, Faculty of Fisheries and Marine Sciences, Riau University. 2019.

Rusli M S. Sukses memproduksi minyak atsiri. AgroMedia. 2010.

Kurniawidjaja L. M. Lestari F. Tejamaya M. & Ramdhan D H. Konsep Dasar Toksikologi Industri. In Fkm Ui. 2021.

Sartika. Ratu Ayu Dewi. Pengaruh Asam Lemak Jenuh,Tidak Jenuh dan Asam Lemak Trans Terhadap Kesehatan. Jurnal Kesehatan Masyarakat Nasional, 2008; Vol.2, No.4, p.154-160

Aspari P. D. Susanti H. Perbandingan Kadar Fenolik Total Ekstrak Metanol Kelopak Merah dan Ungu Bunga Rosella (Hibiscus sabdariffa Linn) secara Spekrpfotometri Prosiding Seminar Nasional Home Care, Fakultas Farmasi dan Kesehatan Masyarakat UAD, Yogyakarta. 2011,

Wahyu D N. Danar D. dan Febriana I. Toksisitas Ekstrak Biji Barringtonia Asiatica (Leecythidaceae) Terhadap Mencit Putih (Mus musculus Strain DDY). Fakultas Pertanian Universitas Padjajaran : Bandung. 2018; ISSN : 2407-7933. https:// journal. uinsgd. ac.id/index.php/ja/article/view/3589

Nurfaat D L. Uji Toksistas Akut Ekstrak Etanol Benalu Mangga (Dendrophtoe petandra) Terhadap Mencit Swiss Webstew. Indonesia Journals of Pharmacheutical Science and Technology, 2016; 3(2), 53-65. ISSN Cetak :2356-1971. ISSN Online : 2406-856X.

Dalimartha S. Atlas Tumbuhan Obat Indonesia Jilid 3, Puspa Swara,Jakarta. 2003.

Idris M. Uji Toksisitas Akut Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) dengan Metode BSLT (Brine Shrimp Lethality Test). Medical Sains : Jurnal Ilmiah Kefarmasian, 2019; 4(1), 35–42. https://doi.org/10.37874/ms.v4i1.121.

Published
2025-11-03
How to Cite
Jamaluddin, J., Ihwan, I., Khaerati, K., Hasanuddin, A., Serdiati, N., Nilawati, J., Wijaya, B., Yuliet, Y., & Ahmad, I. (2025). Acute and Lethal Dose Toxicity Test of Pure Eel Fish (Anguilla marmorata [Q.] Gaimard) Oil in Mice (Mus musculus). Journal of Public Health and Pharmacy, 5(3), 664-677. https://doi.org/10.56338/jphp.v5i3.5987
Issue
Vol. 5 No. 3: NOVEMBER 2025
Section
Articles

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