Household Container Characteristics Associated with Mosquito Larval Presence in a Highland Community of Tana Toraja, Indonesia

Nismawati Natsir (1), A. Arsunan Arsin (2), Hasanuddin Ishak (3), Ilham Alimuddin (4), Masni Masni (5), Muhammad Nasrun Massi (6), Muhammad Syafar (7)
(1) Public Health Study Program, Faculty of Public Health, Universitas Indonesia Timur, Makassar, Indonesia
(2) Department of Epidemiology, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
(3) Department of Environmental Health, Faculty of Public Health, Hasanuddin University, Makassar
(4) Department of Geological Engineering, Engineering Faculty, Hasanuddin University, Makassar, Indonesia
(5) Department of Biostatistics, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
(6) Department of Microbiology Medical faculty, Hasanuddin University, Makassar, Indonesia
(7) Department of Environmental Health, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia
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Natsir, N., Arsin, A. A., Ishak, H., Alimuddin, I., Masni, M., Massi , M. N., & Syafar, M. (2026). Household Container Characteristics Associated with Mosquito Larval Presence in a Highland Community of Tana Toraja, Indonesia. Journal of Public Health and Pharmacy, 6(3), 499–507. https://doi.org/10.56338/jphp.v6i3.10863

Introduction: Dengue continues to pose a major mosquito-borne public health problem in tropical countries, including Indonesia, where household water containers frequently serve as habitats for mosquito larvae. However, studies examining household container characteristics associated with mosquito larval presence in highland communities remain limited.


Methods: A community-based cross-sectional study was conducted in Bombongan Urban Village, Makale District, Tana Toraja Regency, South Sulawesi, Indonesia. The study included 100 households and 128 water-holding containers. Container type, functional container category, container condition, and mosquito larval presence were recorded through direct field observation. Associations were examined using Pearson’s chi-square test and a binary logistic generalized estimating equations (GEE) model to account for household-level clustering.


Results: Of the 128 inspected water-holding containers, 89 (69.5%) were positive for mosquito larvae, and 94 (73.4%) were open. At the household level, 71 of 100 households had at least one larva-positive container, resulting in a House Index of 71.0%, a Container Index of 69.5%, and a Breteau Index of 89. Discarded containers had the highest proportion of larval positivity (91.7%), whereas animal drinking containers were the most frequently observed type. Functional container category was associated with larval presence in the bivariate analysis (p = 0.025) and remained significant as an overall variable in the GEE model (Wald χ² = 12.178, df = 3, p = 0.007), although none of the individual categories differed significantly from animal drinking containers. Open containers had substantially higher adjusted odds of larval presence than closed containers (aOR = 73.56; 95% CI: 21.74–248.87; p < 0.001).


Conclusion: Open container condition was independently associated with mosquito larval presence, whereas functional container category showed a significant overall association. Discarded containers had the highest larval positivity, whereas animal drinking containers were the most frequently observed. Household mosquito-control measures may prioritize covering, emptying, cleaning, and appropriately disposing of water-holding containers.

  1. Brugueras S, Fernández-Martínez B, Martínez-de la Puente J, Figuerola J, Porro TM, Rius C, et al. Environmental drivers, climate change and emergent diseases transmitted by mosquitoes and their vectors in southern Europe: A systematic review. Environ Res. 2020 Dec 1;191. doi: https://doi.org/10.1016/j.envres.2020
  2. Haider N, Hasan MN, Onyango J, Billah M, Khan S, Papakonstantinou D, et al. Global Dengue Epidemic Worsens with Record 14 Million Cases And 9000 Deaths Reported In 2024. International Journal of Infectious Diseases. 2025; 158:107940. doi: https://doi.org/10.1016/j.ijid.2025.107940
  3. Roswati A, Arsin A, Zulkifli A, Amqam H, Khuzaimah A. Spatial analysis and correlation study of dengue hemorrhagic fever in Palopo city, Indonesia. Gac Med Caracas. 2024;132(2). doi: https://doi.org/10.47307/GMC.2024.132.2.13
  4. Arsin AA, Istiqamah SNA, Elisafitri R, Nurdin. Correlational study of climate factor, mobility and the incidence of dengue Hemorrhagic Fever in Kendari, Indonesia. Enferm Clin. 2020; 30:280–4. doi: https://doi.org/10.1016/j.enfcli.2020.06.064
  5. Bagherzadeh F, Hemati S, Mohammadi‐Moghadam F, Sanami S, Salehifard A, Ahmad S, et al. Environmental Determinants of Dengue Fever:‎ A Re‐Emerging Threat in the Middle East. Health Sci Rep. 2025;8(8): e71177. doi: https://doi.org/10.1002/hsr2.71177
  6. Prasad P, Lata S, Gupta SK, Kumar P, Saxena R, Arya DK, et al. Aedes aegypti container preference for oviposition and its possible implications for dengue vector surveillance in Delhi, India. Epidemiol Health. 2023;45: e2023073. doi: https://doi.org/10.4178/epih.e2023073
  7. Ngugi HN, Mutuku FM, Ndenga BA, Musunzaji PS, Mbakaya JO, Aswani P, et al. Characterization and productivity profiles of Aedes aegypti (l.) Breeding habitats across rural and urban landscapes in western and coastal Kenya. Parasit Vectors. 2017;10(1):331. doi: https://doi.org/10.1186/s13071-017-2271-9
  8. Kahamba NF, Limwagu AJ, Mapua SA, Msugupakulya BJ, Msaky DS, Kaindoa EW, et al. Habitat characteristics and insecticide susceptibility of Aedes aegypti in the Ifakara area, south-eastern Tanzania. Parasit Vectors. 2020;13(1):53. doi: https://doi.org/10.1186/s13071-020-3920-y
  9. Souza RL, Nazare RJ, Argibay HD, Pellizzaro M, Anjos RO, Portilho MM, et al. Density of Aedes aegypti (diptera: culicidae) in a low-income Brazilian urban community where dengue, zika, and chikungunya viruses co-circulate. Parasit Vectors. 2023;16(1):159. doi: https://doi.org/10.1186/s13071-023-05766-5
  10. Peña-García VH, LaBeaud AD, Ndenga BA, Mutuku FM, Bisanzio D, Andrews JR, et al. Non-household environments make a major contribution to dengue transmission: implications for vector control. R Soc Open Sci. 2025;12(4). doi: https://doi.org/10.1098/rsos.241919
  11. Acevedo-Guerrero T. Water with larvae: Hydrological fertility, inequality, and mosquito urbanism. Environ Plan E Nat Space. 2022;8(1):13–30. doi: https://doi.org/10.1177/25148486221099801
  12. Vinauger C, Chandrasegaran K. Context-Specific Variation in Life History Traits and Behavior of Aedes aegypti Mosquitoes. Frontiers in Insect Science. 2024; 4:1426715. doi: https://doi.org/10.3389/finsc.2024.1426715
  13. Blanco-Sierra L, Bellver-Arnau J, Escartin S, Mariani S, Bartumeus F. Human–Environment Interactions Shape Mosquito Seasonal Population Dynamics. Insects. 2024;15(7):527. doi: https://doi.org/10.3390/insects15070527
  14. Vannavong N, Seidu R, Stenström TA, Dada N, Overgaard HJ. Effects of socio-demographic characteristics and household water management on aedes aegypti production in suburban and rural villages in Laos and Thailand. Parasit Vectors. 2017;10(1):170. doi: https://doi.org/10.1186/s13071-017-2107-7
  15. Arsin AA, Amiruddin R, Marzuki. Community Empowerment with Independent Larva Monitor in Reducing the Dengue Hemorrhagic Fever Incidence, in Sidrap Regency. Pharmacognosy Journal. 2023;15(4). doi: http://dx.doi.org/10.5530/pj.2023.15.129
  16. Montenegro-Quinonez CA, Louis VR, Horstick O, Velayudhan R, Dambach P, Runge-Ranzinger S, et al. Efficacy of Household Aedes Larval Control Practices an a Peri-Urban Township, Yangon, Myanmar: Implication for Entomological Surveillance. Heliyon. 2023;9(7):104660. doi: https://doi.org/10.1016/j.heliyon.2023.e18083
  17. Ishak H, Toding N, Natsir M, Amqam H. Description of Aedes aegypti Larva Density Based on Mosquito Breeding Eradication Action in the Dengue Endemic Rappocini Sub-District Makassar. In: 3rd International Conference on Environmental Risks and Public Health (ICER-PH 2018). Brussels: EAI; 2019. p. 178–84. doi: http://dx.doi.org/10.4108/eai.26-10-2018.2288868
  18. Dalpadado R, Amarasinghe D, Gunathilaka N. Water quality characteristics of breeding habitats in relation to the density of Aedes aegypti and Aedes albopictus in domestic settings in Gampaha district of Sri Lanka. Acta Trop. 2022; 229:106339. doi: https://doi.org/10.1016/j.actatropica.2022.106339
  19. Sasmita HI, Neoh KB, Ernawan B, Indarwatmi M, Nasution IA, Fitrianto N, et al. Integrated vector management with the sterile insect technique component for the suppression of Aedes aegypti in an urban setting in Indonesia. PLoS Negl Trop Dis. 2025 Jul 1;19(7): e0013290. doi: https://doi.org/10.1371/JOURNAL.PNTD.0013290
  20. Rahman MS, Ekalaksananan T, Zafar S, Poolphol P, Shipin O, Haque U, et al. Ecological, Social, And Other Environmental Determinants f Dengue Vector Abundance in Urban and Rural Areas of Northeastern Thailand. Int J Environ Res Public Health. 2021;18(11):5971. doi: https://doi.org/10.3390/ijerph18115971
  21. Mahmud MAF, Abdul Mutalip MH, Lodz NA, Muhammad EN, Yoep N, Hasim MH, et al. The application of environmental management methods in combating dengue: a systematic review. Int J Environ Health Res. 2023;33(11):1148–67. doi: https://doi.org/10.1080/09603123.2022.2076815
  22. Santana Vieira Santos V, Barbosa Pereira B. Ecotoxicological risks and sustainability of integrated biopesticide-based approaches for Aedes aegypti control. Journal of Toxicology and Environmental Health, Part B. 2025;1–29. doi: https://doi.org/10.1080/10937404.2025.2570725
  23. Soria C, Crocco LB, Grech MG, Stewart-Ibarra A, Almirón WR. Habitat characteristics that favour the presence of Aedes aegypti (Diptera: Culicidae) in households in the city of Córdoba, a temperate area of Argentina. Parasit Vectors. 2025;18(1):487. doi: https://doi.org/10.1186/s13071-025-07114-1
  24. Taslim M, Arsin AA, Ishak H, Nasir S, Usman AN. Diversity of dengue virus serotype in endemic region of South Sulawesi Province. J Trop Med. 2018; 2018:9682784. doi: https://doi.org/10.1155/2018/9682784
  25. Natsir N, Arsin AA, Ishak H, Alimuddin I, Masni M, Massi MN, et al. Transmission of four serotypes of dengue virus in several Asian countries literature review. Pharmacognosy Journal. 2023;15(6):1244–9. doi: https://doi.org/10.5530/pj.2023.15.225
  26. Ishak H, Fiawan MJ, Abdullah MT, Mallongi A. Relationship of the container type and material with the density of Aedes aegypti larvae in Gowa and Maros Regency. Enferm Clin. 2020; 30:399–402. doi: https://doi.org/10.1016/j.enfcli.2019.10.109
  27. South Sulawesi Provincial Health Office. Dengue hemorrhagic fever surveillance report in South Sulawesi Province 2024 [Internet]. Makassar: Dinas Kesehatan Provinsi Sulawesi Selatan; 2025. Available from: South Sulawesi Provincial Health Office archive
  28. Rosser JI, Tarpenning MS, Bramante JT, Tamhane A, Chamberlin AJ, Mutuku PS, et al. Development of a trash classification system to map potential Aedes aegypti breeding grounds using unmanned aerial vehicle imaging. Environmental Science and Pollution Research 2024 31:28. 2024 Jun 6;31(28):41107–17. https://doi.org/10.1007/S11356-024-33801-0
  29. Flaibani N, Pérez AA, Barbero IM, Burroni NE. Different approaches to characterize artificial breeding sites of Aedes aegypti using generalized linear mixed models. Infect Dis Poverty. 2020 Jul 31;9(1):107. https://doi.org/10.1186/S40249-020-00705-3
  30. Velu RM, Kwenda G, Bosomprah S, Chisola MN, Simunyandi M, Chisenga CC, et al. Ecological niche modeling of Aedes and Culex mosquitoes: a risk map for Chikungunya and West Nile Viruses in Zambia. Viruses. 2023;15(9):1900. doi: https://doi.org/10.3390/v15091900
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