Studi Recovery Dutasteride dalam Pelarut Organik terhadap Dutasteride Terlarut dalam Plasma Darah Manusia
Study of Recovery of Dutasteride in Organic Solvents against Dutasteride Dissolved in Human Blood Plasma
Abstract
Latar belakang: Obat Dutasteride tergolong dalam obat golongan keras, yang merupakan obat yang tidak boleh diberikan pada wanita dan wanita hamil memberikan cacat pada bayi laki-laki, merupakan salah satu obat yang dilarang dikonsumsi saat donor darah dan jika seseorang ingin mendonorkan darah harus menghentikan minimal 6 bulan sebelum donor. Belum ada penelitian yang menggunakan Spektrofotometer UV-Vis untuk menganalisis residu obat Dutasteride dalam plasma darah. Pengujian secara kualitatif dan kuantitatif obat Dutasteride dalam plasma darah manusia menggunakan Spektrofotometer UV-Vis dan mencari recovery kadar dari ekstraksi 2 tahap.
Tujuan: Melakukan studi recovery kadar Dutasteride dalam plasma darah dengan metode yang diusulkan, menggunakan Spektrofotometer UV-Vis
Metode: Penelitian ini menggunakan metode eksperimental (bioanalisis) yaitu dengan menggunakan instrumen Spektrofotometer UV-Vis untuk menguji kadar obat Dutasteride secara kualitatif terdapat dalam plasma darah
Hasil: Didapat yaitu recovery total sebesar 64,992%. Ekstraksi dan penambahan reagen BTB dapat menyeleksi senyawa yang tidak dianalisis dalam penelitian ini
Kesimpulan: Studi Kualitatif dan Kuantitatif yang dilakukan menunjukkan bahwa Spektrofotometer UV-Vis dapat digunakan untuk memeriksa kadar 0,006 ppm Dutasteride dan penggunaan reagen BTB dapat berfungsi untuk menyeleksi puncak yang tidak dianalsis.
References
Klikdokter.com. Dutasteride [Internet]. klikdokter. 2020. Available from: https://www.klikdokter.com/obat/obat-saluran-kemih-dan-prostat/dutasteride
My Canadian Pharmacy. Obat-obatan yang Mencegah Anda Mendonorkan Darah Obat Jerawat [Internet]. Csmedcenter.com. 2022. Available from: https://www.csmedcenter.com/medications-preventing-you-from-donating-blood.aspx
Ushirobira CY, Afiune LAF, Pereira MN, Cunha-Filho M, Gelfuso GM, Gratieri T. Dutasteride nanocapsules for hair follicle targeting: Effect of chitosan-coating and physical stimulus. Int J Biol Macromol. 2020;151:56–61.
Hu X, Hu C, Jiang D, Zuo Q, Li Y, Wang Y, et al. Effectiveness of Convalescent Plasma Therapy for COVID-19 Patients in Hunan, China. Vol. 18, Dose-Response. 2020.
Jeong JH, Hyun GH, Park YJ, Kwon SW, Lee AY. Clinical Factors Affecting the Serum Retention of a Teratogenic Etretinate after the Acitretin Administration. Biomol Ther. 2022;30(6):562–9.
Giriraj P, Sivakkumar T. Simultaneous estimation of dutasteride and tamsulosin hydrochloride in tablet dosage form by vierordt’s method. Vol. 10, Arabian Journal of Chemistry. 2017. p. S1862–7.
Madhusudhan P, Reddy MR, Devanna N. Method development and validation of Alfuzosin HCl and Dutasteride in pharmaceutical dosage form by RP-HPLC. Indian J Chem Technol. 2017;24(4):441–6.
Limited TC. TEVA-DUTASTERIDE. Monograph. 2016. p. 1–55.
Pohlman GD, Pohlman EA, David Crawford E. Dutasteride: A review of its use in the management of prostate disorders. Clin Med Insights Ther. 2011;3:171–7.
Kumar A, Saradhi S, Sekaran C, Reddy T. Spectrophotometric Analysis of Dutasteride in Pure and Tablet Dosage Forms. Chem Sci J. 2012;CSJ-47:1–20.
Chaudhari R, Mohanraj K, Shirsat V. MS/MS AND HPLC CHARACTERIZATION OF FORCED DEGRADATION PRODUCTS OF DUTASTERIDE AND TAMSULOSIN HYDROCHLORIDE. IJPSR. 2014;5(7):1–9.
Dwi I, Dan P, Probandari A. Rancangan Penelitian Eksperimental Murni Dan Kuasi-Eksperimental. 2003;164–8. Available from: https://id.scribd.com/document/395605433/Materi-21a-Penelitian-Kuasi-Eksperimental-dan-Eksperimental-pdf
Abbas A. a Validated Tlc- Densitometry for the Simultaneous Determination of Tamsulosin and Dutasteride in Their Combined Pharmaceutical Formulation. Al-Azhar J Pharm Sci. 2021;64(2):93–108.
Deshmukh AV, Shirode AR, Kadam VJ. ANALYTICAL method development and validation for the quantitative estimation of Nimbin in bulk and solid dosage form by RP-HPLC. Int J Pharma Bio Sci. 2021;9(6):120–33.
Patel DB, Patel NJ, Patel SK, Prajapati AM, Patel SA. RP-HPLC Method for the Estimation of Dutasteride in Tablet Dosage Form. Indian J Pharm Sci. 2010;1–6.
Patel DB, Patel NJ, Patel S., Prajapati AM, Patel SA. RP-HPLC method for the estimation of tamsulosin hydrochloride in tablet dosage form. Indian J Pharm Sci. 2010;72(6):785–7.
Prasanna LI, Naidu GT, Fathima N, Chakravathy I., Huq GA. Spectrophotometric Determination of Tamsulosin in the Presence of Dutasteride By Charge Transfer Complex Method By Using 2, 3-Dichloro-5, 6-Dicyano-1, 4-Benzoquinone (Ddq). Int J Res Trends Innov [Internet]. 2018;3(9):6. Available from: www.ijrti.org
Chandrasekhar K, Manikandan A. Novel RP-HPLC method development and validation of Tamsulosin hcl and dutasteride in tablets by ratio’s method. Rasayan J Chem. 2021;14(2):665–71.
Diyya K. Novel stability indicating rp-hplc method development and validation for simultaneous estimation of alfuzosin and dutasteride in pharmaceutical dosage form. Indian J Pharm Educ Res. 2020;54(4):1144–52.
Rames G. T, Prasad YR. Determination of Dutasteride for Analytical Method Development and Validation in Bulk as well as in Pharmaceutical Dosage Form by using RP-HPLC. World J Pharm Sci. 2022;10(9):61–9.
Al-Bayatie YK, Muhamad YH, Kareem ZM, Khathi MT. Determination of dutasteride in pure and tablet by flow injection system. Int J Health Sci (Qassim). 2022;6(March):201–8.
Jati MAS, Febriani H, Kristiani ER. Komparasi Uji Kualitatif Dutasteride Dalam Pelarut Organik terhadap Dutasteride terlarut Dalam Plasma Darah Manusia Menggunakan Spektrofotometri UV-Vis dan HPLC (Literature Review). In: Basic and Applied medical Science Conference (BAMS-C0). 2022. p. 49–56.
Da Silva MHA, Medeiros JL, Costa WS, Sampaio FJB, De Souza DB. Effects of the dutasteride and sildenafil association in the penis of a benign prostatic hyperplasia animal model. Aging Male. 2021;23(5):1009–15.
Afiune LAF, Ushirobira CY, Barbosa DPP, de Souza PEN, Leles MIG, Cunha-Filho M, et al. Novel iron oxide nanocarriers loading finasteride or dutasteride: Enhanced skin penetration for topical treatment of alopecia. Int J Pharm. 2020;587(May).
Noor NM, Umar S, Abdul-Aziz A, Sheikh K, Somavarapu S. Engineered Dutasteride-Lipid Based Nanoparticle (DST-LNP) System Using Oleic and Stearic Acid for Topical Delivery. Bioengineering. 2022;9(1).
Seo SW, Park JW, Han DG, Kim JM, Kim S, Park T, et al. In vitro and in vivo assessment of metabolic drug interaction potential of dutasteride with ketoconazole. Pharmaceutics. 2019;11(12).
Najafizade E, Jamali AK, Zahraei SAH. Clinical Images and Medical Case Reports Unusual presentation of an intracochlear schwannoma. JCIMCR. 2021;2.
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