Studi Recovery Dutasteride dalam Pelarut Organik terhadap Dutasteride Terlarut dalam Plasma Darah Manusia
Study of Recovery of Dutasteride in Organic Solvents against Dutasteride Dissolved in Human Blood Plasma
Abstract
Latar belakang: Obat Dutasteride tergolong dalam obat golongan keras, yang merupakan obat yang tidak boleh diberikan pada wanita dan wanita hamil memberikan cacat pada bayi laki-laki, merupakan salah satu obat yang dilarang dikonsumsi saat donor darah dan jika seseorang ingin mendonorkan darah harus menghentikan minimal 6 bulan sebelum donor. Belum ada penelitian yang menggunakan Spektrofotometer UV-Vis untuk menganalisis residu obat Dutasteride dalam plasma darah. Pengujian secara kualitatif dan kuantitatif obat Dutasteride dalam plasma darah manusia menggunakan Spektrofotometer UV-Vis dan mencari recovery kadar dari ekstraksi 2 tahap.
Tujuan: Melakukan studi recovery kadar Dutasteride dalam plasma darah dengan metode yang diusulkan, menggunakan Spektrofotometer UV-Vis
Metode: Penelitian ini menggunakan metode eksperimental (bioanalisis) yaitu dengan menggunakan instrumen Spektrofotometer UV-Vis untuk menguji kadar obat Dutasteride secara kualitatif terdapat dalam plasma darah
Hasil: Didapat yaitu recovery total sebesar 64,992%. Ekstraksi dan penambahan reagen BTB dapat menyeleksi senyawa yang tidak dianalisis dalam penelitian ini
Kesimpulan: Studi Kualitatif dan Kuantitatif yang dilakukan menunjukkan bahwa Spektrofotometer UV-Vis dapat digunakan untuk memeriksa kadar 0,006 ppm Dutasteride dan penggunaan reagen BTB dapat berfungsi untuk menyeleksi puncak yang tidak dianalsis.
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