Effect of colloidal oatmeal cream on IL-10 and TGF-β levels in UV-B-exposed male Wistar rats
DOI:
https://doi.org/10.56338/ijhess.v8i3.12438Keywords:
Colloidal Oatmeal, IL-10, TGF-β, UV-B, photoagingAbstract
Background: Chronic ultraviolet B (UV-B) exposure causes photoaging and tissue damage through chronic inflammation. Colloidal oatmeal contains avenanthramides and β-glucan with antioxidant, anti-inflammatory, and wound-healing properties, and may enhance interleukin-10 (IL-10) and transforming growth factor-β (TGF-β), which are important for skin repair. This study evaluated the effect of colloidal oatmeal cream on IL-10 and TGF-β levels in UV-B-exposed Wistar rats.
Methods: A post-test-only control group experiment used 36 male Wistar rats in six groups of six: healthy (K1), UV-B with base cream (K2), UV-B with vitamin E cream (K3), and UV-B with 0.5% (K4), 1% (K5), or 2% (K6) colloidal oatmeal cream. Except for K1, rats were exposed to UV-B (70 mJ/cm², 20 cm, 15 minutes daily) for 14 days, and creams were applied twice daily. Skin tissue was collected 24 hours after the last treatment, and IL-10 and TGF-β were measured by enzyme-linked immunosorbent assay.
Results: IL-10 and TGF-β were highest in the 1% group (207.96 ± 24.22 pg/mL and 606.78 ± 50.20 ng/mL). IL-10 in K5 was higher than in K2 (p = .001) and K6 (p = .003), and TGF-β in K5 was higher than in K2 (p = .037), K1 (p = .041), and K6 (p = .045). Collagen thickness did not differ among groups (p = .054). Conclusion: The 1% colloidal oatmeal cream was associated with the highest IL-10 and TGF-β in UV-B-exposed rats, whereas 0.5% and 2% were not.
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